{"id":249,"date":"2024-11-13T19:07:56","date_gmt":"2024-11-13T19:07:56","guid":{"rendered":"https:\/\/ruppweb.org\/psilosophy\/?p=249"},"modified":"2024-11-13T19:07:57","modified_gmt":"2024-11-13T19:07:57","slug":"psid-the-first-step-in-the-making-of-the-magic-chembiochem","status":"publish","type":"post","link":"https:\/\/ruppweb.org\/psilosophy\/?p=249","title":{"rendered":"PsiD: The first step in the making of the Magic -ChemBioChem"},"content":{"rendered":"\n<h1 class=\"wp-block-heading\">Characterization of the Gateway Decarboxylase for Psilocybin Biosynthesis<\/h1>\n\n\n\n<p><a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Sch%C3%A4fer%2C+Tim\">Tim Sch\u00e4fer<\/a>, <a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Kramer%2C+Kristina\">Kristina Kramer<\/a>, <a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Werten%2C+Sebastiaan\">Dr. Sebastiaan Werten<\/a>, <a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Rupp%2C+Bernhard\">Dr. Bernhard Rupp<\/a>, <a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/action\/doSearch?ContribAuthorRaw=Hoffmeister%2C+Dirk\">Prof.\u2005Dr. Dirk Hoffmeister<\/a><\/p>\n\n\n\n<p><a href=\"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.202200551\">First published: 03 November 2022<\/a><\/p>\n\n\n\n<p><strong>How the magic begins<\/strong>: The l-tryptophan decarboxylase PsiD initiates the biosynthesis of the psychotropic natural product psilocybin in <em>Psilocybe<\/em> mushrooms. Its <em>in\u2005vitro<\/em> biochemical characterization and <em>in silico<\/em> modeling of its structure identified a non-canonical serine protease triad for autocatalytic cleavage of the proenzyme. PsiD&#8217;s substrate flexibility makes it a versatile catalyst for biotechnological applications.<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"929\" src=\"https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-1024x929.png\" alt=\"\" class=\"wp-image-195\" style=\"width:623px;height:auto\" srcset=\"https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-1024x929.png 1024w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-300x272.png 300w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-768x697.png 768w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-1536x1394.png 1536w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-512x465.png 512w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-309x280.png 309w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-201x182.png 201w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-169x153.png 169w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-263x239.png 263w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-83x75.png 83w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image-360x327.png 360w, https:\/\/ruppweb.org\/psilosophy\/wp-content\/uploads\/2022\/12\/image.png 1728w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Characterization of the Gateway Decarboxylase for Psilocybin Biosynthesis Tim Sch\u00e4fer, Kristina Kramer, Dr. Sebastiaan Werten, Dr. Bernhard Rupp, Prof.\u2005Dr. Dirk Hoffmeister First published: 03 November 2022 How the magic begins: The l-tryptophan decarboxylase PsiD initiates the biosynthesis of the psychotropic natural product psilocybin in Psilocybe mushrooms. Its in\u2005vitro biochemical characterization and in silico modeling of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-249","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/posts\/249","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=249"}],"version-history":[{"count":1,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/posts\/249\/revisions"}],"predecessor-version":[{"id":250,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=\/wp\/v2\/posts\/249\/revisions\/250"}],"wp:attachment":[{"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=249"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=249"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ruppweb.org\/psilosophy\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=249"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}